QCS 2.38 WINDOWS

1 CuB is recognized as the major active ingredient which possesses a broad range of pharmacological properties such as hepatoprotection, anti-tumor effects and immune enhancement. More than 18 kinds of cucurbitacins can be found in nature, including cucurbitacin B. Also, the absolute bioavailability observed in the present study suggested that it was necessary to change the dosage form to improve bioavailability, or to improve this by other means.Ĭucurbitacins are highly oxygenated, tetracyclic triterpenes that can be isolated from members of the Cucurbitaceae family such as cucumber, watermelon, and zucchini. To sum up, the presented method was determined to be suitable for the quantitation of CuB in rat plasma.

The absolute bioavailability of orally administered CuB in rats was 1.37%. In addition, the stability of the method was also verified. The intra-day and inter-day accuracies and precision deviations were within ☑5%, which was in line with the allowable range of accuracy. The mean extraction recoveries from plasma were 85.34–90.53%. The lower limit of quantification for plasma was 0.05 ng mL −1 the matrix effect of CuB and IS was 94.19–99.42% and 100.83%, respectively. The calibration curves showed good linearity ( r = 0.9998) within the tested concentration ranges. The detection was performed using a multiple reaction monitoring mode via a positive electrospray ionization interface. Separation was achieved on a BEH C18 column (2.1 × 50 mm, 1.7 μm) at 35 ☌ using an isocratic mobile phase system with 0.1% formic acid–acetonitrile (50 : 50, v/v) at a flow rate of 0.3 mL min −1. Plasma samples were processed by liquid–liquid extraction with ethyl acetate. The aim of this study was to develop a method for the determination of CuB in plasma using ultra-performance liquid chromatography-mass spectrometry (UPLC-MS/MS) with estrone as an internal standard (IS), as well as to examine the pharmacokinetics and absolute bioavailability of CuB in rats. Nevertheless, CuB has low solubility and low permeability, and is present at low concentrations in the human body. Cucurbitacin B (CuB) is a highly oxygenated tetracyclic triterpene, and a Biopharmaceutics Classification System (BCS) class IV drug used for the treatment of persistent hepatitis, chronic hepatitis, and primary liver cancer. 2-7Įlements of Quality Control within QCS. 2-3ĬQM Certificate – Sample – Corps of Engineers. 2-2Ĭonstruction Quality Management Training for Contractors.

2-1Ĭontractor Quality Control – Planning. Live Assist / Fast Support / GoToMeeting. Installing QCS for more than one Corps District. 1-38Īn explanation of Site ID’s and Titles. 1-34Įxchanging Data with a Remote QCS Site. Import from RMS into QCS by Exported File. Import from RMS to QCS Using SFTP Server. 1-25Īdding a Contract to the QCS Program. 1-14Ĭontract Menu – Import / Export Screen. 1-12Ĭontract Menu – Quality Control Screen. 1-11Ĭontract Menu – Administration Screen. What makes QCS different from standard commercial programs (COTS)?. HP and LaserJet are registered trademarks of the Hewlett-Packard Corporation.Ĭhapter 1 - Quality Control System Basics.

QCS 2.38 WINDOWS

Windows 2000, Windows NT, Windows XP, Windows VistaĪre registered trademarks of the Microsoft Corporation. Army Corps of Engineers and the author assume no responsibility forĭamages resulting from the use of information contained herein. While every precaution has been taken in the preparation of this manual, Written under contract for the RMS Center,

Qcs 2.38